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Turkish Journal of Fisheries and Aquatic Sciences 2014, Vol 14, Num, 2     (Pages: 547-555)

Recombinant VP28 Produced by Cell-Free Technique Confers Protection in Kuruma Shrimp (Marsupenaeus japonicus) Against White Spot Syndrome Virus

T. Kono 1 ,J. Fall 2 ,H. Korenaga 3 ,R. Sudhakaran 4 ,G. Biswas 5 ,T. Mekata 6 ,T. Itami 1 ,M. Sakai 1

1 University of Miyazaki, Faculty of Agriculture, Department of Biochemistry and Applied Biosciences, 1-1 Gakuenkibanadai-nishi, Miyazaki Japan
2 Institute of Fisheries and Aquaculture, University Cheikh, Austra Diop, UCAD II, Dakar BP5005, Senegal
3 Drug Discovery Platforms Cooperation Division, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan
4 School of Bio-Sciences and Technology, VIT University, Vellore 600 014, Tamil Nadu, India
5 Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan
6 National Research Institute of Aquaculture, Tsuiura, Kamiura, Saiki City, Oita 879-2602, Japan
DOI : 10.4194/1303-2712-v14_2_26 Viewed : 1946 - Downloaded : 1526 The protective efficacy of oral delivery and intramuscular (i.m.) injection of a recombinant protein, rVP28 derived from white spot syndrome virus (WSSV) and synthesized using wheat germ cell-free technology was investigated in kuruma shrimp (Marsupenaeus japonicus). Shrimps were administered with the unpurified rVP28 and challenged with WSSV. Expression of innate immune-related genes was examined in intestine, heart, and lymphoid organ at 1, 3 and 7 days after oraladministration. Shrimps received rVP28 protein through i.m. injection or oral route and then challenged with WSSV displayed higher survival (90 and 85.7%, respectively) compared to the respective control groups. A significant up-regulation (P<0.01) of innate immune-related genes, such as Rab7, penaeidin, lysozyme and crustin, was noticed in orally treated shrimps. Our results indicate that this recombinant protein could elevate immune responses and protection in kuruma shrimp and therefore would have potential utility against WSSV infection in shrimp aquaculture. Keywords : Marsupenaeus japonicus, WSSV, VP28, Innate immune genes, cell-free technique