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Turkish Journal of Fisheries and Aquatic Sciences 2023, Vol 23, Num, 4     (Pages: TRJFAS21811)

Development of a Quantification and Detection Method for 2-MIB-producing Cyanobacteria

Ji Zhang 1 ,Qingyue Shen 1 ,Hanchen Miao 1 ,Qintong Li 2 ,Marie Shimada 3 ,Tian Yuan 4 ,Motoo Utsumi 4-5 ,Zhongfang Lei 4 ,Zhenya Zhang 4 ,Hirokazu Takanashi 6 ,Naoshi Fujimoto 7 ,Satoshi Ichise 8 ,Yasuhiro Asada 9 ,Osamu Nishimura 10 ,Michihiro Akiba 9 ,Kazuya Shimizu 2-4

1 University of Tsukuba, Graduate School of Science and Technology, 1-1-1 Tennoudai, Tsukuba Ibaraki Japan
2 Tokyo University, Faculty of Life sciences, 1-1-1 Izumino Ora-gun Itakura Gunma Japan
3 Water Quality Management Center, Ibaraki Prefectural Public Enterprise Bureau, 2972 Ooiwata, Tsuchiura, Ibaraki, Japan
4 University of Tsukuba, Faculty of Life and Environmental Sciences, 1-1-1 Tennoudai, Tsukuba Ibaraki Japan
5 University of Tsukuba, Microbiology Research Center for Sustainability, 1-1-1 Tennoudai, Tsukuba Ibaraki Japan
6 Kagoshima University, Biotechnology and Chemical Engineering, Department of Chemistry, 1-21-40 Korimoto, Kagoshima City, Kagoshima, Japan
7 Tokyo University of Agriculture, Faculty of Applied Bioscience, 1-1-1 Sakuragaoka, Setagaya-ku Tokyo, Japan
8 Lake Biwa Environmental Research Institute, 5-34 Yanagigasaki, Otsu, Japan
9 National Institute of Public Health, 2-3-6 Minami, Wako, Saitama, Japan
10 Tohoku University, Department of Civil and Environmental Engineering, 6-6-06 Aramaki-Aza Aoba, Sendai, Miyagi, Japan
DOI : 10.4194/TRJFAS21811 Viewed : 1471 - Downloaded : 1132 Consumers often complain about taste and odor (T&O) in drinking water and freshwater fishery. One of the common T&O compounds, 2-methylisoborneol (2-MIB), can be detected by humans even when the concentration is below 10 ng/L. A forecast method of T&O occurrence is required to control drinking water plants and fishery farms to determine the timing of exchange of activated carbon or exchange the water in earthy pond. Traditional monitoring methods such as PCR, microscopy, and chemical analysis require a long time, are high in cost, and have a complex operation. We conducted this study to develop whole-cell PCR and whole-cell qPCR assays for rapid detection and quantification of 2-MIB-producing cyanobacteria without DNA extraction to detect 2-MIB cyclase gene (mtc). Pseudanabaena foetida strain 1705-12 (Lake Kasumigaura), strain 1803-12 (Lake Kasumigaura), and strain PTG (Lake Biwa) of 2-MIB-producing cyanobacteria were used in the study. The positive correlation between the results of whole-cell PCR, whole-cell qPCR and chlorophyll a (Chl.a), and gene abundances illustrated that whole-cell PCR and whole-cell qPCR assays could rapidly and conveniently detect and quantify 2-MIB-producing cyanobacteria. Thus, this study provides a valuable tool for prediction of T&O events in drinking water and freshwater fishery. Keywords : 2-MIB Whole-Cell PCR Whole-cell qPCR Pseudanabaena foetida Phormidium tenue