Turkish Journal of Fisheries and Aquatic Sciences
2017, Vol 17, Num, 1 (Pages: 153–169)
Molecular Response of Carbohydrate Metabolism to Dietary Carbohydrate and Acute Low Salinity Stress in Pacific White Shrimp Litopenaeus vannamei
Xiaodan Wang 1 ,Erchao Li 1 ,Zhixin Xu 1 ,Tongyu Li 1 ,Chang Xu 1 ,Liqiao Chen 1
1 East China Normal University, School of Life Sciences, Laboratory of Aquaculture Nutrition and Environmental Health, Shanghai, 200241, China
DOI :
10.4194/1303-2712-v17_1_18
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Molecular response of carbohydrate metabolism in Litopenaeus vannamei was evaluated at transcriptional level through
quantitative real-time PCR analysis. The mRNA expressions of six genes hexose-6-phosphotransferase, pyruvate kinase,
phosphoenolpyruvate carboxykinase, crustacean hyperglycemic hormone, glucose transporter and insulin-like growth factors
binding protein in the hepatopancreas, muscle, gill and eyestalk were analyzed in shrimp fed different levels of carbohydrate
and challenged with acute salinity stress. Among these genes, the relative expression of GLUT1, CHH and IGF-BP in the
muscle was lower or nearly the same as the control group regardless of the dietary carbohydrate level. The relative
expressions of HK and PK were much higher in the hepatopancreas, muscle, gill and eyestalk at 12 h after salinity stress. The
relative expression of PEPCK showed a down-up-down tendency and the expression was much higher in different tissues
(except in eyestalk) than in the control from 12 to 24 h after stress. This study indicates that there was no significant dietary
carbohydrate regulation in muscle. The glycogen and amino acid in muscle were the main source energy for osmoregulation
in L. vannamei when the animals were challenged with acute salinity stress. The muscle and gills were the main
osmoregulation organ in shrimp under hypo-saline stress.
Keywords :
Litopenaeus vannamei, carbohydrate metabolism, dietary carbohydrate, acute salinity stress, molecular response