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Turkish Journal of Fisheries and Aquatic Sciences 2016, Vol 16, Num, 3     (Pages: 571-578)

A Novel PCR Detection Method for Major Fish Pathogenic Bacteria of Vibrio anguillarum

Shotaro Izumi 1 ,Kyuma Suzuki 2

1 Course of Applied Biological Science, Department of Fisheries, School of Marine Science and Technology, Tokai University, Orido, Shimizu, Shizuoka, 424-8610, Japan
2 Gunma Prefectural Fisheries Experimental Station, Shikishima, Maebashi, Gunma 371-0036, Japan
DOI : 10.4194/1303-2712-v16_3_09 Viewed : 4382 - Downloaded : 3680 For the rapid identification and detection of Vibrio anguillarum, we have PCR amplification technique targeting gyrB region has been evaluated. We have designed twosets of PCR primers for the specific amplification of gyrB in V. anguillarum using single and nested PCR. PCRs specificity was demonstrated by successful amplicon from V. anguillarum DNA. The detection limit of the single PCRs and the nested PCR were 4.0 pg and 40 fg of V. anguillarum DNA, respectively. Using the nested PCR, the direct sensitive detection of V. anguillarum from organs of diseased fishes is possible. Keywords : Vibrio anguillarum, PCR detection, gyrB