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5-Year Impact Factor: 1.4
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UN SDG
Turkish Journal of Fisheries and Aquatic Sciences 2011, Vol 11, Num, 4     (Pages: 615-622)

Micronucleus Test, Nuclear Abnormalities and Accumulation of Cu and Cd on Gambusia affinis (Baird & Girard, 1853)

Utku Güner 1 ,Fulya DilekGökalp Muranlı 1

1 Trakya University, Faculty of Sciences, Department of Biology, 22030, Edirne, Türkiye DOI : 10.4194/1303-2712-v11_4_16 Viewed : 4384 - Downloaded : 4794 In the present work the induction of micronuclei (MNi) and nuclear abnormalities (NAs) in erythrocytes and Cu and Cd accumulation in whole body of Gambusia affinis were studied. Fish were exposed to two different Cu and Cd concentrations, 0.1 ppm and 1 ppm, for 1 and 2 weeks periods and to Cu-Cd combination (0.1 ppm Cu + 0.1 ppm Cd) for 2 weeks period using a semi-static renewal system. Micronucleus and nuclear abnormality analysis were carried out on peripheral blood erythrocytes. When fishes were exposed Cu and Cd in combination, Cu accumulation was increased compared to their singly (0.1 ppm) exposed concentrations. Micronucleus and nuclear abnormality analysis tests revealed that, although Cu and Cd did not significantly increase micronuclei frequency, nuclear abnormalities were significantly induced compared to control groups. Keywords : Gambusia affinis, nuclear abnormalities, heavy metal accumulation, Cu, Cd.