Turkish Journal of Fisheries and Aquatic Sciences
2011, Vol 11, Num, 3 (Pages: 377-383)
Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration
Tolga Göksan 1 ,İlknur Ak 1 ,Cenker Kılıç 1
1 Çanakkale Onsekiz Mart University, Faculty of Fisheries, Department of Aquaculture, 17020, Çanakkale, Turkey
DOI :
10.4194/1303-2712-v11_3_06
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In the present study, the effects of various inorganic nitrogen sources, light intensities, aeration rates and especially
vitamins were investigated in vegetative Haematococcus pluvialis Flotow cultures. The best growth among the nitrogen
sources was achieved in NaNO3 (1.0 g/L) and KNO3 (0.5 g/L) with cell densities of 25.3 and 26.3x104 cells/ml respectively.
In vitamin trials, the highest cell numbers were found in 0.1 (0.3 μM), 1.0 (4 μM) and 0.1 mg/L (0.75 μM) concentrations for
thiamin, biotin and B12 respectively. It was also seen that the use of thiamin alone was sufficient instead of using the vitamin
mix. Cell growth was significantly higher in 75 and 150 μmol photon m-2 s-1 illuminations compared to 20 and 40 μmol
photon m-2 s-1. As for aeration, optimum airflow rate was 1.0 lpm, while supra-optimum levels ceased the growth, cell
diameter and pigment content increased. So, it may play a role as a secondary stress factor on H. pluvialis cells in reddening
stage.
Keywords :
Çanakkale Onsekiz Mart University, Faculty of Fisheries, Department of Aquaculture, 17020, Çanakkale, Turkey