Turkish Journal of Fisheries and Aquatic Sciences
2011, Vol 11, Num, 2 (Pages: 253-259)
Comparative Studies on Toxicokinetics and Residues of Dimethyl Phthalate (DMP) in Tilapia(Oreochromis niloticus × O. aureus)at Different Water Temperatures
Jian Ge 1 ,Mingkui Li 1 ,Fang Lin 1 ,Jin Zhao 1 ,Xianjun Dai 1
1 China JiLiang University, College of Life Sciences, Hangzhou, Zhejiang, 310018, China
DOI :
10.4194/trjfas.2011.0210
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The study was conducted to evaluate the toxicokinetic disposition and residue of Dimethyl phthalate (DMP) in tilapia at
different water temperatures (18ºC and 28ºC) by RP-HPLC method. After oral administration of DMP with a single does of
20mg/kg body weight, the tilapia’s plasma, muscle, brain, skin and myocardium were sampled separately at different time to
determine the concentration of DMP. Then the data were analyzed using 3p97 Pharmacokinetics software written by the
Chinese Society of Mathematical Pharmacology, which indicated that the absorption, distribution and elimination half-life at
28ºC were 0.020 h, 0.19 h and 40.16 h, while the absorption half-life and elimination half-time were 3.01 h and 57.62 h at
18ºC. That’s to say, the DMP absorption rate was faster in tilapia at 28ºC than that at 18ºC. And the serum concentration-time
curve (AUC) was 30.13μg h ml-1 at 28ºC, while the T-peak was 0.074 hrs and the C-peak were 2.06μg·ml-1; whereas, the
corresponding situations at 18ºC were 74.77 μg·h·ml-1, 13.51 hrs and 0.76μg·ml-1, respectively. No matter how, the DMP
residue was detected after oral administration for 360 hrs at both water temperatures, but the research had revealed that water
temperature had great impact on the DMP absorption, distribution and elimination in tilapia.
Keywords :
Tilapia( Oreochromis niloticus×O.aureus, Dimethyl phthalate (DMP), RP-HPLC, toxicokinetics, residue