Turkish Journal of Fisheries and Aquatic Sciences
2012, Vol 12, Num, 3 (Pages: 555-564)
Low Genetic Variation Suggest Single Stock of Kawakawa Euthynnus affinis (Cantor, 1849) along the Indian Coast
Girish Kumar 1 ,Swaraj Priyaranjan Kunal 1 ,Maria Rosalia Menezes 1
1 National Institute of Oceanography, Dona Paula, Goa 403004, India
DOI :
10.4194/1303-2712-v12_3_02
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Kawakawa Euthynnus affinis is an epipelagic migratory tuna species, widely distributed in the tropical and subtropical
waters of the Indo-Pacific region. It constitutes the largest tuna fishery in Indian waters. In present study, restriction fragment
length polymorphism (RFLP) analysis of the mitochondrial D-loop region was employed to examine the levels of genetic
diversity among kawakawa samples collected from eight main fishing zones (Veraval (VE), Ratnagiri (RA), Kochi (KO),
Kavaratti (KA), Port-Blair (PB), Tuticorin (TU), Pondicherry (PO) and Vizag (VI)) along the Indian coast. A 500 bp segment
of mitochondrial D-loop region was screened in 400 samples using six restriction enzymes (Rsa I, Alu I, Hinf I, Hha I, Msp I
and Hae III), resulting in 13 composite morphs. Analysis of molecular variance (AMOVA) of mtDNA data revealed no
significant genetic differentiation among sites (FST = -0.00446, P = 0.84946). Results of the genetic analyses of present study
suggest the single stock of kawakawa along the Indian coast.
Keywords :
PCR-RFLP, mtDNA, D-loop, Indian waters