Turkish Journal of Fisheries and Aquatic Sciences
2012, Vol 12, Num, 4 (Pages: 861-869)
Investigations on the ATPase Activities and Cadmium Uptake in Freshwater Fish Oreochromis niloticus Following Exposures to Cadmium in Increased Salinity
Berna Kulac 1-2 ,Gülüzar Atli 1 ,Mustafa Canli 1
1 Çukurova University, Faculty of Science and Letters, Department of Biology 01330, Balcali, Adana, Turkey
DOI :
10.4194/1303-2712-v1241_14
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Anthropogenic activities can increase the salinity of freshwaters. Thus, freshwater fish Oreochromis niloticus were
exposed to Cd (1.0 μg/mL) in increased salinities (0, 2, 4 and 8 ppt) for 0, 1, 3, 7 and 14 days. Following single and combine
exposures to salinity and Cd, Na+/K+-ATPase, Mg2+-ATPase, Ca2+-ATPase activities were measured in the gill, kidney and
intestine. In general, salinity alone exposures increased Na+/K+-ATPase activity in the gill and intestine while there were
fewer alterations in the kidney. Mg2+-ATPase activity significantly altered in the intestine and kidney. Ca2+-ATPase in the
tissues significantly decreased, the kidney showing the most decrease. Salinity+Cd combine exposures decreased Na+/K+-
ATPase activity in 2 ppt medium in the gill while the activity increased at 8 ppt medium. Na+/K+-ATPase activity in the
intestine decreased in relation to salinity increase, though there was no significant decrease in the kidney. Mg2+-ATPase and
Ca2+-ATPase activities showed a declining trend with the increase in salinity. Cd accumulation in the tissues decreased as the
salinity of medium increased, though accumulation in the gill increased regardless of salinity increase at the longest exposure
period. Results emphasized necessity of measuring fish ATPase activities in evaluation of data from environmental
monitoring, as they are highly sensitive to the changes in the aquatic medium.
Keywords :
ATPase, tilapia, metal, osmoregulation, gill, kidney, intestine