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Turkish Journal of Fisheries and Aquatic Sciences 2022, Vol 22, Num, 10     (Pages: TRJFAS19877)

The Effects of Bisphenol A on Oxidative Stress, Antioxidant Defence, Histopathological Alterations and Lysozyme Activity in Narrow-Clawed Crayfish (Pontastacus leptodactylus)

Öznur Diler 1 ,Öznur Özil 1 ,İkbal Demet Nane 1 ,Mevlüt Nazıroğlu 1 ,Mert Minaz 2 ,Rahime Aslankoç 3 ,Özlem Özmen 4 ,Kâmil Atsatan 1

1 Isparta University of Applied Sciences, Faculty of Eğirdir Fisheries, Aquaculture Department, Isparta, Turkey
2 Recep Tayyip Erdoğan University, Faculty of Fisheries and Aquatic Sciences, Aquaculture Department Rize, Turkey
3 Süleyman Demirel University, Faculty of Medicine, Department of Physiology, Isparta, Turkey
4 Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, Burdur, Turkey
DOI : 10.4194/TRJFAS19877 Viewed : 175 - Downloaded : 164 This study aimed to investigate of bisphenol A (BPA) on histopathological tissue (hepatopancreas and gills), immune ability (lysozyme activity), oxidative stress and antioxidant defence in crayfish (Pontastacus leptodactylus). Crayfish were exposed to BPA to various concentrations (0 (control), and 10, 50, 100 μg/L) for 5 and 20 days in triplicates. Histopathological findings showed moderate to severe hyperemia and inflammatory cell infiltrations, irregular epithelial cell arrangements, degeneration, necrosis and sloughing at the cells, collapse of the hepatopancreas tubules and hyperemia, oedema and sloughing at the epithelial cells of gills. In addition to swelling of gills, accumulation of hemocytes in the hemocoelic space of the gill lamellae relating to dosage were noticed. Antioxidant-related enzyme activities which superoxide dismutase (SOD), Glutathione-S-transferase GST, and glutathione reductase (GR) activity in 10, 50, 100 μg/L BPA group were significantly lower than control at 5 days (p<0.05). Lysozyme activity (LSZ) was no significantly enhanced according to control (p>0.05). These results suggest that the alterations in the antioxidant enzymes and the histological structure of the hepatopancreas, and gill tissue of crayfish can be used as potential biomarkers for risk assessment in aquatic ecosystem. Keywords : Crayfish histopathology oxidative stress antioxidant defence BPA