Turkish Journal of Fisheries and Aquatic Sciences
2021, Vol 21, Num, 12 (Pages: 615-625)
In Silico Bioactive Peptide Prediction from The Enzymatic Hydrolysates of Edible Seaweed Rubisco Large Chain
Ayse Kose 1
1 Ege University Faculty of Engineering Department of Bioengineering, 35100, Bornova, Izmir, Turkey
DOI :
10.4194/1303-2712-v21_12_04
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Seaweeds are one of the ancient food supplements on Earth. Especially Asian countries
use seaweeds as the fundamental ingredient in their cuisine. Seaweeds are
photosynthetic organisms living in aquatic ecosystems and in the coastal territories.
Seaweeds out of farm areas are frequently observed as coastal wastes. However,
seaweeds are outstanding sources for bioactive substances and investigation bioactive
properties of seaweed RuBisCO has never been done. RuBisCO is the most abundant
protein on Earth but a vast amount of RuBisCO goes through waste. In this study,
bioactive peptide prediction of frequently consumed seaweed RuBisCO proteins were
analyzed in silico to identify possible bioactive peptides as substitute or support for
grain, meat, and dairy based bioactive peptides. A huge portion of peptides were di-,
tri- peptides with IC50 values less than 300 µM according to the comparison of BIOPEP
database. Including gastric digestion, more than half of the peptides showed DDP-IV
and ACE inhibitory activity followed by antioxidant properties. Also, novel antiinflammatory and anti-cancer peptides were found through in silico analysis.
Keywords :
Bioactive peptides Functional foods, In silico, Macroalgae, RubisCO