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Turkish Journal of Fisheries and Aquatic Sciences 2021, Vol 21, Num, 12     (Pages: 603-613)

Using Inter Simple Sequence Repeat Multi-Loci Markers for Studying Genetic Diversity in Guppy Fish

Mohammadreza Mohammadabadi 1 ,Valentyna Oleshko 2 ,Olexandr Oleshko 2 ,Leonid Heiko 2 ,Iryna Starostenko 3 ,Jurii Kunovskii 2 ,Alevtina Bazaeva 4 ,Zahra Roudbari 5

1 Shahid Bahonar University of Kerman, Faculty of Agriculture, Department of Animal Science, Kerman, Iran
2 Bila Tserkva National Agrarian University, Department of Aquaculture, Bila Tserkva, Ukraine
3 Bila Tserkva National Agrarian University, Department of Animal Science, Bila Tserkva, Ukraine
4 National University of Life and Environmental Sciences of Ukraine, Department of Hydrobiology and Ichthyology
5 University of Jiroft, Faculty of Agriculture, Department of Animal Science, Jiroft, Iran
DOI : 10.4194/1303-2712-v21_12_03 Viewed : 1534 - Downloaded : 1906 Aquarium fish industry has become a capacity for job creation and income generation. Aquarium fish uses for decoration and creating tranquility in the environment and beautification in public places. This study was conducted to investigate genetic variation between different populations of various varieties of guppy ornamental aquarium fish using inter simple sequence repeat (ISSR) markers in Iran for the first time. DNA was extracted from twelve strains. Polymerase chain reaction was performed using seven ISSR primers. GenAlex 6.501 and XLSTAT software was used to analyze data. Results of analysis of molecular variance showed that 54% of observed polymorphism belongs to variance among populations and 46% corresponds to diversity between populations. The highest and the lowest polymorphism percentage belonged to (GAG)5GC (90%) and (AG)8C (41%) markers, respectively. Percentage of polymorphic loci ranges from 32.31% to 86.15%. The results of phylogenetic tree showed that this tree was divided into 2 major clusters. Cluster I included only 2 populations and cluster II contained other 10 studied populations. It is suggested that this marker system was found to be efficient in discriminating each genotype at the molecular level and can be used for genetic diversity analysis for aquarium guppy fish because a greater understanding of the potential of the species is necessary for supporting long-term genetic improvement. Keywords : Aquarium fish industry, Phylogenetic tree, Polymorphism, ISSR markers