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Turkish Journal of Fisheries and Aquatic Sciences 2020, Vol 20, Num, 7     (Pages: 521-529)

Cloning and Expression of Two Carboxylesterases, and Their Activity Modulation in Chinese Mitten crab Eriocheir sinensis under Pesticide Exposer

Yanan Zang 1 ,Xiao Zhang 1 ,Pengdan Huang 1 ,Kassimu Hashim Ame 1-2 ,Huaishun Shen 1-2

1 Nanjing Agricultural University, Wuxi Fisheries College, Nanjing, 210095, PR China
2 Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Wuxi 214081, PR China
DOI : 10.4194/1303-2712-v20_7_02 Viewed : 2338 - Downloaded : 5880 Carboxylesterases (CXEs) belong to a family of multifunctional enzymes. They metabolize drugs, environmental toxicants, and carcinogens, and inhibit bacterial pathogenesis. In this study, the full-length cDNAs of ES-CXE3 (2,444 bp) and ES -CXE4 (2,385 bp) were cloned from the Chinese mitten crab, Eriocheir sinensis. Sequence analysis showed that both ES-CXE sequences contained the catalytic triplet structure characteristic of the CXEs superfamily. Alignment and phylogenetic analyses revealed that the two ES-CXEs are highly similar to those of other crustaceans. Tissue specificexpression analysis showed that both ES-CXEs were highly expressed in the hepatopancreas. Real-time fluorescence quantitative PCR showed that the maximum expression levels of the ES-CXE3 and ES-CXE4 genes in the hepatopancreas of E. sinensis exposed to low doses of β-cypermethrin, avermectin and trichlorfon were 10, 8, 6 and 600, 110, 250 higher than relative to those of the control group, respectively, and that enzyme activities steadily increased and were significantly higher than that of the control group. Therefore, treatment with these insecticides may induce the expression of both ES-CXEs as well as changes in the activities of carboxylesterase family genes. Our results suggest that ES-CXEs might play vital roles for insecticide detoxification in E. sinensis Keywords : Carboxylesterases, cDNA clone, Chinese mitten crab, Insecticide detoxification