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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 2     (Pages: 175-178)

Development of Twenty-Two Novel Cross-Species Microsatellite Markers for Amur sturgeon (Acipenser schrenckii) from Chinese Sturgeon (Acipenser sinensis) via next-Generation Sequencing

Yacheng Hu 1 ,Xueqing Liu 1 ,Jing Yang 1 ,Kan Xiao 4 ,Binzhong Wang 1 ,Hejun Du 1

1 Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Institute of Chinese Sturgeon, China Three Gorges Corporation, Yichang, Hubei, 443100, China DOI : 10.4194/1303-2712-v19_2_10 Viewed : 3113 - Downloaded : 2369 In the present study, twenty-two microsatellites was developed for Amur sturgeon (Acipenser schrenckii). All of them were polymorphic with 2 to 7 alleles per locus and the total number of alleles is 106. The expected heterozygosity (HE), observed heterozygosity (HO), polymorphic information content (PIC), Hardy-Weinberg departure value (d) and Shannon-Weiner Diversity Indices (H') ranged from 0.35 to 0.84, from 0.46 to 1, from 0.29 to 0.82, from 0.14 to 0.49, and from 0.53 to 1.89 respectively. The microsatellites described here should be useful for the research in population genetics, conservation genetics and evolution of Amur sturgeon. Keywords : Microsatellite, Cross-species, Acipenser schrenckii, Genetic diversity