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Turkish Journal of Fisheries and Aquatic Sciences 2017, Vol 17, Num, 3     (Pages: 477-485)

Peroxisome Proliferator-Activated Receptor Gamma (Pparγ) in Redlip Mullet Liza haematocheila:Molecular Cloning, Tissue Distribution, and Response to Dietary Lipid Levels

Aimin Wang 1 ,Wenping Yang 1-2 ,Fei Liu 1 ,Xiaoling Yin 1 ,Yebing Yu 1

1 College of Marine and Bioengineering, Yancheng Institute of Technology, Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Yancheng, China
2 Key laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China
DOI : 10.4194/1303-2712-v17_3_04 Viewed : 3738 - Downloaded : 3518 Redlip mullet (Liza haematocheila) PPARγ cDNA (lhPPARγ) by reverse transcription polymerase chain reaction (RT - PCR) and rapid amplification of cDNA ends (RACE) were isolated in this study. The full-length cDNA was 2985 bp, consisted of a 200 bp 5’UTR, 1183 bp 3’UTR and 1599 bp ORF encoding a polypeptide with 533 amino acids. lhPPARγ protein was predicted to consist of four domains, i.e. N-terminal domain, DNA-binding domain, C-terminal ligand binding domain and flexible hinge domain. Real time quantitative RT-PCR revealed that lhPPARγ mRNA was detected in all tested tissues. The highest expression level of lhPPARγ mRNA was observed in abdomen fat followed by intestine, gill, kidney, liver, skin, brain, spleen, stomach, liver, heart and muscle. These results suggested that lhPPARγ was functionally and evolutionarily conserved between redlip mullet and other vertebrates. Six iso-nitrogenous diets with different lipid level (from 2.0% to 14.6%) were fed to triplicate groups. Although fish fed diets containing 14.6% lipid showed higher lhPPARγ mRNA expression in the liver, intestine and muscle than fish fed other diets, no significant difference was observed, which indicated redlip mullet might adapt to high lipid level below 14.6%. Keywords : Liza Haematocheila, PPARγ, Cloning, Expression, Lipid