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¹ College of Food Science & Technology, Huazhong Agricultural University Wuhan, 430070, P. R. China
² Key Laboratory of Environmental Correlative Dietology, Ministry of Education Wuhan, 430070, P. R. China
³ Agricultural Research Institute Tarnab Peshawar, Pakistan
⁴ Key Laboratory of Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University Wuhan, 430070, P. R. China
⁵ Food Technology Centre, Pakistan Council of Scientific and Industrial Research Labs. Peshawar, Pakistan DOI : 10.4194/1303-2712-v17_1_16 Viewed : 4125 - Downloaded : 3343 Contamination of aquatic ecosystems with toxic metals such as Cd²⁺ and Pb²⁺ is a serious issue in the industrialized world, which can affect freshwater fish even at low concentrations. The aim of the present study was to investigate the effect of Cd²⁺ and Pb²⁺ alone or in combination on the biochemical constituents of liver and muscle in Crucian carp using Fourier Transform Infrared Spectroscopy (FT-IR). Results from the spectral analysis revealed significant decline in protein and increase in lipids in the two tissues with marked effect caused by the combined exposure of Cd²⁺ and Pb²⁺. In case of liver, alteration in the intensity and band area at amide I resulted in a differential response of structural protein for the exposed groups. Furthermore, a decrease in the α-helix and alterations in the nucleic acids content was also observed in both liver and muscle of the exposed fish. Moreover, biochemical alterations in the vital tissue of freshwater fish due to toxic contaminants can be used as a marker of environmental pollution with the help of FT-IR spectroscopy. Keywords : FT-IR, Crucian carp (Carassius auratus gibelio), Liver, Muscle, Cd2+, Pb2+, Biochemical changes