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¹ Adnan Menderes University, Faculty of Agriculture, Department of Aquacultural Engineering, Güney Kampüsü 09100, Aydın, Turkey DOI : 10.4194/1303-2712-v16_3_13 Viewed : 8702 - Downloaded : 5276 The present experiment was designed to determine the effects of tricaine methanesulfonate (MS-222) as an anaesthetic on goldfish Carassius auratus at five different salinities and MS-222 concentrations. Goldfish (230±25 mg and 24.07±5.59 mm) were exposed to 150, 200, 300, 400, and 500 mg l-1 MS-222 concentrations at 0, 8, 12, 14, and 16 ppt of salinities. Even though, a lot of literature exist about the anesthetic usage on foodfish species, not much information seems to be available on ornamental fish aquaculture. Five tricaine methanesulfonate concentrations for each salinity were used to anesthetize goldfish and recorded their induction and recovery times. The necessary time to make anesthesia on fish relied on concentration intensity and salinity. When exposed to any of the concentrations, fish achieved a deep state of anesthesia (range of induction time 0.13 and 8.90 min). It is recommended that ideal concentration of MS-222 was 200 mg l-1 at 12 ppt to reduce fish mortality and stress. Keywords : Carassius auratus, goldfish, salinity, tricaine methansulfonate