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¹ College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan DOI : 10.4194/1303-2712-v16_3_03 Viewed : 4240 - Downloaded : 3355 The biological activities of water-soluble extracts obtained from the flatfish (Pleuronectes obscurus) tail fin which is a waste material generated by the fish processing industry were investigated in this study. The flatfish extract inhibited lipid accumulation in 3T3-L1 preadipocyte cells during their differentiation in a concentration-dependent manner. Furthermore, it downregulated the expression of peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α, which are the key transcription factors, involved in adipocyte differentiation. Additionally, a differentiationinhibiting glycoprotein with a molecular weight of approximately 30-kDa from the flatfish extract was isolated. These results suggest that several glycoproteins in the flatfish extract, particularly purified 30-kDa glycoprotein, inhibit adipocyte differentiation by suppressing the expression of PPAR γ and C/EBP α. Keywords : Adipogenesis, fish waste, flatfish, glycoprotein, tail fin