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¹ Çukurova University, Fisheries Faculty, Balcalı, Adana, Turkey DOI : 10.4194/1303-2712-v15_2_00 Viewed : 2489 - Downloaded : 2322 In this research, in aquatic environments negative effects which may occur in order to have information about, the acute toxicity Fluazifop-P-Butyl (herbicide) which widely used in agriculture in Çukurova Region has been researched into larvae Oreochromis niloticus (L., 1754).In this study, the test fish O. niloticus larvae (average weight 1.88±0.6 g, average length 5.04±0.5 cm) were obtained from the Çukurova University Fisheries Faculty Freshwater Fish Research and Application Station. In the experiment, five different concentrations together with the control group were used total 12 aquariums. Each experiment were repeated for two times. In the present study, methods of bioassay test which called static method has been applied. The study was carried out under laboratory conditions at 25±2 oC. The results has been estimated with the doseresponse data were fitted a log-logistic model by using R 3.0 statistical computation environment and DRC library. The 24-h acute LC50value were calculated. According to the results, acute toxic effects researched Fluazifop-P-Butyl (herbicide), the 24-h LC50 acute toxic lethal concentration value for Oreochromis niloticus (L., 1754)larvae was calculated to be 1.94±0.02 mgl-1.These results indicate that low levels of Fluazifop-P-Butyl(herbicide) in the aquatic environment may have a significant effect on the O. niloticus populations. Keywords : Bioassay, Herbicide, Fluazifop-P-Butyl, Oreochromis niloticus, LC50