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¹ University of Warmia and Mazury in Olsztyn, Dept. Lake and River Fisheries, ul. Oczapowskiego 5, PL 10-718, Olsztyn-Kortowo, Poland
² University of Lodz, Department of Applied Ecology, Poland
³ European Regional Centre for Ecohydrology, under the auspices of UNESCO, Polish Academy of Sciences, Lodz, Poland
⁴ Polish Academy of Science, Institute of Animal Reproduction and Food Research, Dep Gamete and Embryo Biology, Olsztyn, Poland
⁵ National Medicines Institute, Warsaw, Poland DOI : 10.4194/1303-2712-v15_2_34 Viewed : 4019 - Downloaded : 4097 This study presents the results of inducing spermiation and ovulation of dace (Leuciscus leuciscus L.) spawners collected from different parts in of Poland. Artificial reproduction was performed using carp pituitary homogenate (CPH), human chorionic gonadotropin (hCG), agents containing a GnRHa with a dopamine inhibitor supplement (Ovopel and Ovaprim, GnRHa combined with metoclopramide) and GnRHa alone conducted during the spawning season. Males from the control group produced similar volumes of semen and sperm motility as fish after hormonal preparation application. Stimulation of ovulation by manipulation of environmental factors as well as by GnRHa alone did not trigger ovulation within 70 hrs following administration of a resolving injection, but did cause GV migration. Applying GnRHa with the dopamine inhibitors or CPH produced 80-100 % ovulation in females. The differences in reproduction between fish populations were reflected in ovulation synchronization, which was highly variable among populations or embryo survival. The fish stimulated with CPH ovulated several hours earlier than those injected with GnRHa combined with a dopamine inhibitor. The data obtained in the present paper could be applied to the production of the rheophilic cyprinid stocking process and, consequently, could form an important tool in the conservation of endangered or over-exploited finfish populations. Keywords : Artificial reproduction; rheophilic cyprinids; wild populations; CPH; GnRHa; hCG