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¹ Gorgan University of Agricultural Science and Natural Resource, Fisheries Faculty, P.O. Box: 45165-386, Gorgan, Iran DOI : 10.4194/1303-2712-v11_4_13 Viewed : 4246 - Downloaded : 6206 The objective of this study was to assay the effectiveness injection of different hormones such as ovaprim, human chorionic gonadotropin (hCG) and carp pituitary gland (cPG) on spermatological parameters and seminal plasma composition of cultured and wild carp. At the dosage tested, motility duration, percentage of motile spermatozoa, spermatocrit and sperm volume were significant changes (P<0.01) in both stimulated groups and treatments. Composition of seminal plasma (Na⁺, K⁺, Cl-, glucose, cholesterol and protein) were significant changes in cultured and wild carp, but values of Ca⁺² and Mg⁺²did not show significant difference (P<0.01) in treatments. In general, significant changes in all treatments for spermatological properties and composition of seminal plasma were observed. The results showed that ovaprim and carp pituitary gland hormones had more effective than human chorionic gonadotropin (hCG) in cultured and wild carp. Keywords : Hormone treatment, Spermatological parameters, Seminal plasma, Cultured and Wild Carp