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¹ Annamalai University, Department of Zoology, Annamalainagar 608 002, Tamil Nadu, India Viewed : 8025 - Downloaded : 5484 The pattern of zinc (Zn) residue in various tissues of Channa punctatus exposed to two treatments of zinc (6.62 and 13.24 mg L⁻¹) for a period of 45 days was analysed. The concentrations of Zn was found to follow the order of liver > kidney > intestine > gill > muscle. Zn accumulated significantly (P<0.05) in all the tissues was studied except muscle tissue. Present findings revealed that liver and kidney are the prime sites of Zn accumulation and zinc load in the muscle was for low as compared to other organs. In the muscles of C. punctatus, 4.95 and 5.29 μg Zn g⁻¹ was accumulated in fish exposed to lower and higher sublethal concentration of Zn at 45th day of exposure, respectively. These values were low as compared to 49.72 and 67.31 μg Zn g⁻¹ of accumulated level in prime site of liver tissue of test fish exposed to lower and higher sublethal concentration of zinc, respectively. Further, a linear relationship of Zn accumulation with exposure concentrations was noted in all tissues of test fish. These data will constitute a reference for future studies on the evaluation of Zn accumulation tendency in relation to the ecotoxicological testing scheme for hazard assessment. Keywords : zinc, Channa punctatus, residues, organs, long-term exposure