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¹ Central Fisheries Research Institute, Vali Adil Yazar St. No:14, Kaşüstü, Yomra, Trabzon, Turkey DOI : 10.4194/1303-2712-v12_3_15 Viewed : 4172 - Downloaded : 4859 The aim of this study wasto determine the optimal dose and the time required to reach a stable level of anaesthesia using clove oil and benzocaine on Siberian sturgeon fry (Acipenser baerii Brandt, 1869) with a mean length of 10.0±0.93 (7.2-12.0) cm and weight of 4.1±0.95 (2.1-6.4) g, and to compare the effects of them on feed intake after anaesthesia. In addition, the survival time and relationship between exposure duration and recovery time in 96 min with the optimal concentrations of these two anaesthetics were examined as part of the anaesthesia procedure. Effective concentrations (EC₅₀) were estimated to be 356 (330-381) mg/L of clove oil and 37 (33-40) mg/L of benzocaine for 3 minutes with 95% confidence limits. The recovery time with clove oil was longer than with benzocaine at 6, 12, 24 and 48 minutes (P<0.05). The mean lethal times (MLT₅₀) were estimated as 37.2 (5.1-69.3) min for clove oil and 63.0 (6.1-120.0) min for benzocaine with 95% confidence limits. Feed intake in fish treated with clove oil and benzocaine occurred within ten minutes, but the effect of both anaesthetics on feed intake continued for 4 h. However, it was determined that the effect of clove oil on feed intake was less than that of benzocaine. Keywords : Siberian sturgeon, Anaesthesia, clove oil, benzocaine, feed intake