Abstract Full Text:PDF Recommended articles Mail to Author How to Cite

¹ Karadeniz Technical University, Faculty of Marine Science, Department of Marine Sciences and Technology Engineering, Surmene, Trabzon, Turkey
² Recep Tayyip Erdoğan University, Department of Fisheries and Aquatic Sciences, Rize, Turkey DOI : 10.4194/1303-2712-v14_3_06 Viewed : 4957 - Downloaded : 5028 Present study was conducted to determine the effects of long term carbosulfan exposure on erythrocyte and liver acetylcholinesterase (AChE) activity in rainbow trout (Oncorhynchus mykiss), and to assess sensitive tissue to carbosulfan in terms of AChE activity. For these purpose, fish were allowed to recover in toxicant-free water for 24 days after 60 days of exposure. AChE activity was determined spectrophotometrically using acetylthiocholine iodide as substrate in the erythrocyte and liver. Erythrocyte and liver AChE of carbosulfan-exposed fish showed considerable inhibition rate. A higher degree of enzyme inhibition was observed in the erythrocyte when compared with liver. The degree of enzyme inhibition had a positive correlation with the time of exposure. Erythrocyte and liver AChE activities were recovered after 18 d and 21 d, respectively. Results indicate that the erythrocyte AChE activity is more sensitive to carbosulfan exposure than that of liver AChE. The greater sensitivity of the erythrocyte AChE suggests that it may be more useful as a biomarker of carbosulfan exposure or contamination. Keywords : Enzyme activity, biomarker, pesticide, liver protein, Oncorhynchus mykiss