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¹ Karadeniz Technical University, Faculty of Marine Science, Department of Fisheries Technology Engineering, 61530 Surmene, Trabzon, Turkey DOI : 10.4194/trjfas.2010.0103 Viewed : 4507 - Downloaded : 4247 The acute toxicity of ammonium sulphate, calcium ammonium nitrate and two different composite fertilizers [15:15:15 (NPK-1) and 25:5:10 (NPK-2)] to juvenile rainbow trout (Oncorhynchus mykiss; 0.89±0.22 g) was evaluated under semistatic conditions. The concentrations of ammonium sulphate, calcium ammonium nitrate, NPK-1 and NPK-2 that killed 50% of the rainbow trout within 96-h (96-h LC50) were 0.149, 0.259, 0.258 and 0.408 g/L, respectively. The mortality of fish was elevated with increasing concentrations of the fertilizers. The time required to kill 50% (LT50) of the rainbow trout at the highest concentrations tested was 11 h 18 min for ammonium sulphate, 12 h 23 min for calcium ammonium nitrate, 13 h 38 min for NPK-1 and 17 h 47 min for NPK-2. Ammonium sulphate was the most toxic compound to rainbow trout compared to composite fertilizers. Keywords : fertilizer, NPK, acute toxicity, rainbow trout