Turkish Journal of Fisheries and Aquatic Sciences
2003, Vol 3, Num, 2 (Pages: 131-138)
Molecular Diagnosis of Fish Diseases: a Review
İlhan Altinok 1 ,İlknur Kurt 1
1 Karadeniz Technical University, Sürmene Faculty of Marine Sciences, Department of Fisheries Technology Engineering, 61530 Surmene, Trabzon, Turkey
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Molecular techniques are potentially faster and more sensitive than culture, serology, and histology methods that are
traditionally used to identify fish pathogens. During the last 15 years or so, molecular techniques have been increasingly
employed to diagnose fish diseases. These techniques include polymerase chain reaction (PCR), restriction enzyme digestion,
probe hybridization, in situ hybridization, and microarray. Pathogens can be detected from asymptomatic fish by molecular
diagnostic techniques so disease outbreak could be prevented. Thus antibiotic treatment can be reduced so that creation of
antibiotic resistant bacteria may be eliminated. In this paper molecular techniques for detection of fish pathogens are
reviewed and the potential for their application are discussed. The application of new techniques as a routine tool in a
diagnostic laboratory is an area where relevant literature is scarce and this may contribute to the reticence of some to adopt
these methods.
Keywords :
Molecular techniques, fish diseases, antibiotic resistance, molecular diagnosis