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¹ Krishna University, Department of Biotechnology, Machilipatnam, 521001, AP, India
² PG Courses, P.G Department of Biotechnology, Research Center, DNR College, Bhimavaram, 534202, Andhra Pradesh, India DOI : 10.4194/1303-2712-v13_4_21 Viewed : 4370 - Downloaded : 3495 This communication provides an insight in to the emerging of new infection fulminant sepsis in Pangasius sutchi and aimed to screen prime pathogens involved in disease. The pathogen was isolated from diseased P. sutchi characterized by morphological, biochemical and molecular approach, which includes 16s r RNA gene sequencing. PCR amplified 16s RNA was separated using agarose gel electrophoresis, eluted product was sequenced and blast analysis was carried out to identify the pathogen. Wohlfahrtiimonas chitiniclastica with LD₅₀ dose 10^8.35 has been initiated re-infection in experimentally infected Pangasius fingerlings. This study provides the evidence of newly emerged Wohlfahrtiimonas chitiniclastica which was true causative agent of fulminant sepsis in Pangasius. There was no track record of Wohlfahrtiimonas chitiniclastica fulminant sepsis infection in P. sutchi till date around globe. To the best of knowledge, this is the first report of Wohlfahrtiimonas chitiniclastica infection in Pangasius sutchi. Keywords : Pangasius sutchi, 16s r RNA gene sequencing, Wohlfahrtiimonas chitiniclastica, challenge test