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¹ Department of Marine Bioscience and Technology, Gangneung-Wonju National University, Gangneung 210-702, South Korea DOI : 10.4194/1303-2712-v13_4_15 Viewed : 4963 - Downloaded : 4722 This research was carried out to investigate the effects of dietary inclusion of distillers dried grain (DDG) level on growth performance, feed utilization, body composition and antioxidant enzyme activity of juvenile black seabream (Acanthopagrus schlegeli). Five isonitrogenous and isocaloric diets were formulated that contain 0 (control), 6%, 12%, 18% and 24% DDG designated as DDG0, DDG6, DDG12, DDG18 and DDG24, respectively. Three replicate groups of fish averaging 1.2 ± 0.01 g were fed with one of the experimental diets for visual satiety three times a day for 8 weeks. Weight gain was not affected by dietary DDG level (P>0.05). Feed efficiency and protein efficiency ratio of fish fed with DDG24 diet were lower than those of fish fed DDG0 diet (P<0.05). Proximate and amino acid composition of whole body were not affected by dietary DDG level. The activities of superoxide dismutase and glutathione peroxidase in the liver were not affected by dietary DDG level (P>0.05). The experiment suggested that DDG is a good ingredient to replace plant origin such as wheat flour and gluten meal and could be used up to 24% for the optimum growth performance of juvenile black seabream. Keywords : Dietary ingredient, distillers dried grain, Black seabream