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¹ Anhui Academy of Agricultural Sciences, Fisheries Research Institution, Anhui Key Laboratory of Aquaculture and Stock Enhancement, Hefei, China, 230031Institute of Fisheries Science, Anhui Academy of Agricultural Sciences, Nongke SouthRoad, Hefei, Anhui, China DOI : 10.4194/TRJFAS25574 Viewed : 44 - Downloaded : 33 Culter alburnus is one of the most important commercial freshwater fishes and widely distributed in China. In this study, a total of 31 novel single nucleotide polymorphism (SNP) markers were developed based on whole-genome resequencing. The analysis of the 31 SNP genotypes in 60 fish showed that the observed heterozygosity and expected heterozygosity ranged from 0.283 to 0.650 and 0.280 to 0.504, respectively. The minor allele frequency ranged from 0.167 to 0.458, and the polymorphism information content ranged from 0.239 to 0.375. Among these SNPs, only one SNP were found to deviate from Hardy-Weinberg equilibrium significantly (P<0.01). These SNP markers could be a valuable tool for population conservation in this species. Keywords : Topmouth culter, SNP markers, Population genetics, Whole genome resequencing