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¹ Pathology Division, Aquaculture Research Department, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-Ise, Mie 516-0193, Japan
² Miyako Field Station, Aquaculture Research Department, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 4-9-1 Sakiyama, Miyako, Iwate 027-0097, Japan DOI : 10.4194/TRJFAS25671 Viewed : 228 - Downloaded : 134 Currently, little is known about vertical transmission of cyprinid herpesvirus 3 (CyHV-3). Here, we assessed potentials for (1) vertical transmission of CyHV-3 and (2) viral infection to fertilized eggs via viral-contaminated environmental water. After CyHV-3 exposure, eggs were incubated, and hatchlings were raised to juvenile stage. To assess occurrence of KHVD during the experiment, samplings were performed at various stages, such as fertilized egg, eyed egg, larval, and juvenile stages. The viral DNA was detected only from eggs at 3 h post fertilization, but not from fish at later stages. No mortality associated with CyHV-3 infection was observed in all the groups during the experimental period, or fish became 91 days post hatch. Additionally, those juveniles were re-exposed to CyHV-3 to investigate whether CyHV-3 infection was established at the egg stage. As a result, statistically significant difference was not observed in the cumulative mortality between the larval fish with or without CyHV-3 infection at the egg stage, suggesting that fish exposed to CyHV-3 at the egg stage did not acquire immunity against the viral infection. All our results suggest that it is improbable that KHVD can be transmitted vertically through eggs of common carp that have survived CyHV-3 infection. Keywords : Cyprinid herpesvirus 3 Cyprinus carpio Koi herpesviral disease Vertical transmission