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¹ Department of Fisheries Hydrography, College of Fisheries, Shirgaon, Ratnagiri (Dr.B.S.K.K.V. Dapoli), Maharashtra- 415629
² Department of Aquaculture, College of Fisheries, Shirgaon, Ratnagiri (Dr.B.S.K.K.V. Dapoli), Maharashtra- 415629 DOI : 10.4194/TRJFAS24608 Viewed : 654 - Downloaded : 567 An experiment of acute toxicity was conducted to find out the lethal concentration 50 (LC₅₀) of chloropyrifos (CPF) for Pangasianodon hypophthalmus. Our findings indicated that the 96-h LC50 value of CPF for P. hypophthalmus was 0.106 mg L^-1. Effects of different concentrations of CPF (0.09, 0.1, 0.11, 0.12, and 0.13 mg L^-1) for 96-h on hemato-biochemical, enzyme and histological effects were evaluated in this study. Acute exposure induced significant differences in enzymes acid phosphatase, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase and acetylcholine of blood serum of the treated group as compared to control group. However, significant decreases in serum total protein, globulin, albumin, and triglycerides levels while serum glucose enhanced with consequent increasing doses of CPF exposure. Also, CPF induced significant declines in RBC count, haemoglobin and hematocrit level whereas the exposure groups showed significant rises in WBC and blood indices levels as compared to control group. Furthermore, CPF exposure causes gill curling, epithelium lifting, necrosis, and lamellae degeneration. Hemosiderin, sinusoid dilations, necrosis, malanomacrophage, hepatocyte hypertrophy were observed in liver histology. Reduction of lumen tubule, hyperplasia, increased bowman space and degenerations were seen in kidney. Overall findings indicates that the acute CPF exposure to P. hypophthalmus resulted in significant adverse changes in biochemical, hematological, and histopathological response. Keywords : Chlorpyrifos Acute toxicity Hematology Biochemical Histology