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¹ Department of Fisheries & Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
² Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, Turkey.
³ Department of Animal Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan.
⁴ Aquaculture and Nutrition Research Unit (ANRU), Carna Research Station, Ryan Institute and School of Natural Sciences, University of Galway, Carna, Connemara, Co. Galway, H91 V8Y1, Ireland
⁵ Aquaculture and Nutrition Research Unit (ANRU), Room 204, Annex Building, Ryan Institute and School of Natural, University of Galway, Galway City, H91 TK33, Ireland DOI : 10.4194/TRJFAS22204 Viewed : 2165 - Downloaded : 1806 The present study was conducted to evaluate the effect of different dietary protein levels on digestive enzyme activities, nutrient assimilation as well as growth performance in striped catfish (Pangasianodon hypophthalmus). Fish were fed with four semi-purified formulated diets with increasing levels of protein: 30% (CP30), 35% (CP35), 40% (CP40) and 45% (CP45) for 12 weeks. At the end of the feed trial, the highest weight gain (29.88 g fish-1), specific growth rate (3.58%) and lower feed conversion ratio (1.91) were observed in fish that were fed with a 40% protein diet while CP30 treatment group had the poorest growth performance indicators (P<0.05). Proximate analysis revealed that fish fed with either CP40 (18.50%) or CP45 (19.33%) diets had the highest protein content in comparison to that fed CP30 (17.13%) and CP35 (17.00%) diets. Similarly, the highest body lipid content was CP35 (4.20%) treatment group and the lowest in CP40 (3.83%). The amino acid in the harvested fillets was also significantly higher (P<0.05) in both CP40 and CP45 dietary treatments. The increasing dietary protein level also elevated both protease and amylase activity, while lipase activity significantly decreased (P<0.05). The study revealed that a 40 % protein level diet produced from regional ingredients in the Middle East had yielded optimal growth performance and higher muscle quality in striped catfish. Keywords : Striped Catfish Pangasianodon hypophthalmus Growth Perfromance Amino Acid Profile Digestive Enzyme activity