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¹ Bogor Agricultural University, Faculty of Fisheries and Marine Sciences, Department of Aquatic Product Technology, Bogor 16680, Indonesia DOI : 10.4194/TRJFAS19268 Viewed : 1332 - Downloaded : 1402 Adhesion of biofouling organism creates various problems. Efforts to explore the source of the antifouling material from marine organisms have been carried out, but it is non-cultivable mainly; its work efficiency depends on temperature, pH, concentration, and ineffective exposure time. Therefore, it is necessary to explore new sources of antifouling material by developing antifouling biocomposites from chitosan-ZnO. This study aims to determine the effect of differences in concentration of chitosan and ZnO on biocomposite characterization. This study consisted of 3 stages, 1) characterization of chitosan and ZnO; 2) production and characterization of chitosan-ZnO biocomposite; and 3) antifouling activity testing. The results showed that chitosan was completely soluble in 2% acetic acid with a viscosity of 76.4 to 79.6%, a water content of 10.92%, ash of 1.92%, nitrogen of 3.70%, and a deacetylation level of 85%. The ZnO used in this study had a particle size of 396.1-458.7 nm. Biocomposite characteristics indicated that the best treatment was chitosan 1% -ZnO 0.6 g with 0.31% swelling, 0.18% solubility film, 104.50° hydrophobic and 4.50 MPa adhesion. The results of the anti-fouling tests showed that the treatment of chitosan 1% -ZnO 0.6 g had less biofouling than other treatments. Keywords : Chitosan ZnO Biocomposite Biofouling Antifouling