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¹ Lahore Garrison University, Department of Biology, Sector C phase 6 DHA Lahore, Pakistan
² Government College University, Department of Zoology, Microbiology Lab, Lahore, Pakistan
³ Univeristy of Karachi, Department of Microbiology, Karachi, Pakistan
⁴ College of Forestry Hainan University Haikou, 570228, China
⁵ Hainan University Haikou, College of Ecology and Environment, 570228, China DOI : 10.4194/TRJFAS21768 Viewed : 1977 - Downloaded : 2452 Waste materials are of major concern around the globe. This study aimed to utilize the scales of fish (Labeo rohita) in microbe-mediated gelatin extraction. Three bacterial isolates were isolated from the fish scales and one bacterial isolate FSW3 showing maximum gelatin yield (61.4 %) was subjected to further analysis. The bacterial culture FSW3 (identified as Staphylococcus spp.) with enhanced production of extracted gelatin was set for physiochemical testing, FTIR analysis, UV-Vis spectroscopy, and stereomicroscopic analysis. The extracted gelatin was successfully utilized in various applications including plant growth promotion under salt stress, anti-adhesion films, firmness of yogurt, biodegradable plastic, and anti-microbial food coatings. Results showed that 0.01 mM gelatin promoted plant growth promotion under salt stress. FSW3 extracted gelatin decreased the 2-fold biofilm-forming ability of E. coli, when exposed to the gelatin-based anti-adhesion films coated on a glass slide and polystyrene sheet. The gelatin showed promising findings as anti-microbial food coatings for tomatoes preservation after 21 days and helpful in the firmness of yogurt. Another advantage of gelatin was casting it into biodegradable plastic. The current study provides a promising approach for fish scales microbe-mediated gelatin extraction and uses it to explore new avenues in biotechnology and microbiology. Keywords : Anti-adhesion Anti-microbial coatings Bacterial isolates Biodegradable plastic Salt stress scales