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Turkish Journal of Fisheries and Aquatic Sciences 2020, Vol 20, Num, 12     (Pages: 889-899)

Determining the Dose of Dietary Probiotic (Bacillus licheniformis) for the Nursing of Blue Swimming Crablets (Portunus pelagicus, L., 1758)

Anawat Boonyapakdee ,Ram Chandra. Bhujel 1

1 Aquaculture and Aquatic Resources Management, Department of Food, Agriculture and Bio-resources, School of Environment, Resources and Development, Asian Institute of Technology, 58 Moo 9, Km. 42, Paholyothin Highway Klong Luang, Phatum Thani, 12120, Thailand DOI : 10.4194/1303-2712-v20_12_05 Viewed : 1986 - Downloaded : 2554 Various efforts have been made to use probiotics instead of antibiotics in aquaculture to solve the problem of low survival and slow growth of hatchlings. Selection of probiotics strain and their right dose have been the major challenges. Limited research has been done in blue swimming crab (Portunus pelagicus L., 1758). Therefore, present study was carried out to determine the effects of varying doses of probiotic Bacillus licheniformis 107 colony forming unit (CFU)/mL i.e. 0.0, 1.5, 3.0, and 6.0 mL/kg on the survival, growth and moulting of blue swimming crablets nursed for 45 days and determine the right dose. The four treatments were replicated thrice in 500 L plastic tanks. Regression analysis revealed that the probiotic dose had significant (P<0.05) positive relationships with carapace length gain, weight gain, and moulting percentage; but negative relationship with food conversion ratio. Vibrio colony growth was significantly (P<0.05) inhibited at the dose of 3.3 mL/kg feed or higher. Results showed that increased doses of Bacillus licheniformis enhanced the growth and moulting of blue swimming crablets. The highest moulting percentage of 89 % was found at an extrapolated dose of 6.9 mL/kg feed. Keywords : Nursing Blue Swimming Crab, Crablet Nursing, Probiotics, Bacillus licheniformis Dose Effect, Growth and Moulting