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¹ Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China
² Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, P.R. China
³ University of Chinese Academy of Sciences, Beijing 10049, P.R. China DOI : 10.4194/1303-2712-v20_6_02 Viewed : 2594 - Downloaded : 1703 The nanos gene family plays a critical role during germline development in a wide array of organisms. The characteristics of the gene structure and function of nanos1, a nanos homolog, vary from species to species. Herein, we isolated the full-length cDNA of a nanos1 homolog in the olive flounder, Paralichthys olivaceus, and analyzed its expression pattern. The full-length cDNA sequence of the olive flounder nanos1 has 1215 base pairs (bp) and contains a 112 bp 5′-untranslated region, a 684 bp open reading frame that encodes a 228 AA peptide, and a 419 bp 3′- untranslated region. Phylogenetic analyses showed that the Nanos1 homolog of the olive flounder grouped with the nanos1 of teleost fish. We detected nanos1 mRNA in all stages of embryogenesis using RT-PCR analyses. Our whole mount in situ hybridization results showed that nanos1 was expressed in the diencephalon, midbrain, hindbrain, nose, medulla oblongata, retina, abdomen, and somatic gonadal cells. Our data indicate that the expression pattern of nanos1 is not consistent from species to species, supporting that nanos1 had different functions in different organisms. Keywords : Abdomen, Gonads, Phylogenetic analysis, Teleost fish