Turkish Journal of Fisheries and Aquatic Sciences
2020, Vol 20, Num, 5 (Pages: 359-366)
Interaction between Kisspetin and Dopamine in the Regulation of in vitro LH Release in Prussian carp (Carassius gibelio Bloch, 1782) Females at the Time of Gonad Recrudescence and Spawning Period
Mirosława Sokołowska-Mikołajczyk 1 ,Grzegorz Gosiewski 1 ,Jarosław Chyb 1 ,Magdalena Socha 1
1 Department of Ichthyobiology and Fisheries, University of Agriculture, ul. Prof. T. Spiczakowa 6, 30-199 KrakowMydlniki, Poland
DOI :
10.4194/1303-2712-v20_5_04
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The aim of the present study was to demonstrate the in vitro response (LH release) of
Prussian carp pituitary cells to mammalian kisspeptin in static incubations. Pituitary
glands were collected at two periods of the season (at the time of gonad
recrudescence or spawning period) from untreated fish or those with dopaminergic
system blocked in vivo by injection of pimozide - a dopamine receptor antagonist. LH
measurement (by ELISA method) have shown that there was no significant impact of
kisspeptin on the level of this gonadotropin at both investigated seasons of the year if
pituitary glands for the study were collected from untreated females. In case of fish
pre-treated with pimozide there was a significant stimulation of LH release in both
investigated seasons. The results demonstrate the direct effect of kisspeptin on LH
release from the Prussian carp gonadotropic cells, dependent on the strength of
dopamine inhibition on LH secretion.
Keywords :
Dopamine, Human kisspeptin, LH, Prussian carp
