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¹ Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Vietnam DOI : 10.4194/1303-2712-v19_12_03 Viewed : 4050 - Downloaded : 3306 Silver nanoparticles (AgNPs) have been widely used to produce consumer goods that vary in type and application. Undoubtedly the increase of production and consumption of these silver-containing products has led to the entry of silver compounds into the aquatic system. In this study, we used tropical groups of aquatic organisms including the freshwater green algae Scenedesmus acuminatus, the marine diatom Chaetoceros gracilis and the microcrustacean Daphnia lumholtzi as model organisms to investigate the acute and chronic toxicity of AgNPs. The test organisms were exposed following specific and standardized protocols for each species/endpoints, with modifications when necessary. The endpoints recorded were growth inhibition, survival, growth of parent animals and number of live neonates produced. The EC50 values of AgNP for the growth inhibition of S. acuminatus and C. graciliswere 38.5 μg/L and 24.3 μg/L, respectively. The values of median lethal concentrations (24h- and 48 h-LC50) of AgNPs to D. lumholtzi were 69.3 and 57.6 μg/L. In the chronic exposure, tested animals were exposed to AgNP from 0.1 to 5 μg/L for 21 days. Chronic effects of AgNP on survival, growth and reproduction of the microcrustacean D. lumholtzi were observed in concentrations higher than 0.5 μg/L. Keywords : Acute and chronic tests, Algae growth inhibition, Aquatic organisms