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¹ Gansu Agricultural University, College of Animal Science and Technology, 1 Yingmencun, Anning District, Lanzhou, 730070, Gansu, China
² Qingyang Academy of Agricultural Sciences, Qingyang, 745000, Gansu, China DOI : 10.4194/1303-2712-v19_8_06 Viewed : 3750 - Downloaded : 2987 Rainbow trout (Oncorhynchus mykiss) is a cold-water species, and often affected by high temperatures. To understand hsp30 mRNA expression trend and molecular mechanism responding to high temperature stress, real-time qPCR was used to quantify hsp30 mRNA expression level in different tissues (heart, liver, gill, brain, spleen and head kidney) in control (18°C) and heat-stressed (25°C for 2, 4, 8 and 12 h) groups. Results showed that hsp30 mRNA in all tissues had already synthesized at 18°C. hsp30 mRNA expression in all tissues was significantly up-regulated and reached the peak after heat stress at 25°C for 2-4 h (P<0.05), then significantly down-regulated at 8 h (P<0.05), and finally returned to control level at 12 h and HSR (P>0.05). The highest hsp30 mRNA expression levels were recorded in heart and liver after 2-4 h. However, the most significant changes of hsp30 mRNA after 2-4 h were observed in gill by 15668.243- and 11452.125-fold compared with 18°C. These results indicated that hsp30 mRNA expression in rainbow trout was induced by heat stress and responded in a time- and tissue-specific manner. Hsp30 can provide some protection in heat stress, but it is only within a certain range. Keywords : Cold-water species, High temperatures, Small heat shock proteins, Tissue-specific gene expression