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¹ Soochow University, School of Biology & Basic Medical Science, Suzhou 215123, China
² Aquaculture Technical Extension Station of Kunshan City, Suzhou 215300, China DOI : 10.4194/1303-2712-v19_8_02 Viewed : 3121 - Downloaded : 2714 In recent years, an epizootic causing severe mortality among the Allogynogenetic crucian carp (ACC), designated as haemorrhagic disease of ACC gill, occurred in the Yancheng city of Jiangsu province of China. A herpesvirus, Cyprinid herpesvirus 2 (CyHV-2) was found in diseased ACCs and regarded as a pathogen, but the host range and vertical transmission of CyHV-2 remained unknown. In present study, we conducted loop-mediated isothermal amplification (LAMP) assay, PCR and real-time PCR to detect CyHV-2 in the eggs of diseased Carassius auratus, and specific products could be amplified from the extracted DNAs from eggs of the diseased fish. RT-PCR result indicated that helicase gene of CyHV-2 could express in eggs. Moreover, the CyHV-2 virus-like particles, ranged between 170 to 220 nm in diameter, could be observed by electron microscopy in eggs of the diseased fish. These results suggested that CyHV-2 probably transmitted vertically to offspring. Additionally, instead of the usual host, the goldfish, Carassius auratus and Carassius auratus gibelio, CyHV-2 was also detected in Aristichthys nobilis, Erythroculter ilishaeformis, Hypophthalmichthys molitrix and Mylopharyngodon piceus with gill haemorrhagic disease characterized by LAMP assay and electron microcopy examination. Therefore, our experimental results indicated that CyHV-2 can cross-infection among the different species of fishes. Keywords : Cyprinid herpesvirus 2 (CyHV-2), Host range, Vertical transmission; molecular detection, Electron microscopy