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¹ University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II 389 25 Vodnany, Czech Republic DOI : 10.4194/1303-2712-v19_03_09 Viewed : 3427 - Downloaded : 3421 The survival of grass carp juveniles overwintered in ponds and RAS were compared in this study. The grass carp, W = 2.49 ± 0.34g, were distributed among five 350 L tanks connected to a RAS and/or into three (1500 m2) ponds (P group). After 165 days, the survival rate was significantly higher in the RAS group, 97.8 ± 0.5%, compared to 10.9 ± 11.4% in P group. The grass carp in a RAS during the first winter significantly improved survival and good conditions of juvenile for subsequent ongrowing period. The RAS provided production profit compare to loss in P group. Keywords : RAS, Pond culture, Overwintering, Cyprinids, Fish production