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¹ China Jiliang University, Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, Hangzhou, China DOI : 10.4194/1303-2712-v18_11_06 Viewed : 5497 - Downloaded : 4228 Microalgae have been widely used in aquaculture as feedstuff and feed additive. The aim of this research was to isolate and identify the microalgae derived from a pool used to breed soft-shell turtles. Therefore, the biomass and total sugar content under different light and temperature conditions were investigated. Using morphological and molecular identification tools, the microalgae were identified as Botryococcus sp. The optimal culture conditions in terms of both the growth and the total sugar content of Botryococcus sp. were a culture temperature of 28℃ and an illumination intensity of 65 μmol m−2 s−1 in BG11 medium; in its stable stage, the biomass of Botryococcus sp. could reach 0.86 g/L and its total sugar content, 35.6% (dry weight). Total sugar accumulation was positively correlated with the activities of glucose phosphate isomerase and hexokinase, and Botryococcus polysaccharide was composed of L-(+)-rhamnose, D-ribose, D-arabinose, D-xylose, 2-deoxyD-glucose, D-mannose, D-glucose, D-galactose and N-Acetyl-D-glucosamine. Keywords : Freshwater microalgae, identification, optimization of growth conditions, polysaccharide synthase, monosaccharide composition