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¹ University of Warmia and Mazury in Olsztyn, Department of Fish Biology and Pisciculture, Oczapowskiego 5, PL 10-719 Olsztyn, Poland
² National Marine Fisheries Research Institute, Department of Logistics and Monitoring, Kołłątaja 1, PL 81-332 Gdynia, Poland DOI : 10.4194/1303-2712-v18_5_10 Viewed : 3526 - Downloaded : 2328 This study presents a protocol of immersing sea trout Salmo trutta m. trutta L. larvae within an alizarin red S (ARS) solution to provide highly visible single and double fluorescent marks in their otoliths. The 14 day interval and temperature regime between the first and second batch marking sessions have been found to develop the proper distance between the ARS marks in the otoliths. The mean values of increment widths between the first and second ARS marks in the otoliths of sea trout marked twice within 14 day period ranged between 21.11–34.98 μm, depending on the year of the experiment. The distance between ARS marks corresponded to temperature during the period between first and second fish immersions which was 4.4ºC, 7.5ºC and 6.7ºC, for year 2014, 2015, and 2016 respectively. As a result, this method can be used as a routine procedure that provides clear and well-visible single and double alizarin marks in the otoliths and helps to distinguish fish originating from different stocking groups of wild-born individuals. Keywords : salmonids, fluorochromes, alizarin red S, increment widths, Łeba River