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¹ Jenderal Soedirman University, Faculty of Biology, Jl. Dr. Suparno 63, Purwokerto 53123, Central Java, Indonesia DOI : 10.4194/1303-2712-v18_5_04 Viewed : 4237 - Downloaded : 4719 Spirulina platensis (S. platensis) is a blue-green algae, shaped like a spiral, contains high nutritional value including proteins, vitamins, minerals and essential amino acids. The objective of this study is to evaluate the effects of supplementation S. platensis in diets and to get the level of supplementation S. platensis in diets gurami fish, Osphronemus gouramy best promote growth, hematological, body composition and biochemical blood parameters. The fish are used to measure the average weight 2.52 ± 0.17 g. The treatments tested was 5 groups, replicated 3 times for each group. The dietary levels S. platensis of five treatment group were: C (control group), P1, P2, P3, P4, respectively supplementation S. platensis 0, 2, 3, 4, and 6 g/kg of diet, the trial period is 56 days.The results showed that the level difference S. platensis supplementation can increase growth, hematological and biochemical blood parameters gurami. Best growth, body composition, hematological and biochemical blood parameters of gurami is obtained at the level of supplementation S. platensis 6 g/kg of diet. Findings from this study indicate that supplementation S. platensis 6 g/kg of diet in aquaculture gurami increasing growth, body composition and immunity body fish. Keywords : Immunity, Osphronemus gouramy, Spirulina platensis, supplementation