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¹ Gyeongsang National University, Department of Seafood and Aquaculture Science, Tongyeong 53064, Republic of Korea
² Pusan National University, School of Medicine, Department of Parasitology, Yangsan-si Gyeongsangnam-do 626-870, Republic of Korea DOI : 10.4194/1303-2712-v18_4_08 Viewed : 5503 - Downloaded : 3832 Two experiments were conducted to investigate the effects on growth, survival and anti-inflammatory cytokine (Interleukin-10) using with Nannochloropsis concentration in diets of sea cucumber Apostichopus japonicus. In the first experiment, 60 days feeding trail was conducted to evaluate the growth performance and survival of the sea cucumber fed on six experimental diets containing different inclusion level of Nannochloropsis oculata (0%, 2%, 4%, 6%, 8%, 10%) in recirculating aquaculture system. Specific growth rate (SGR) and food conversion efficiency (FCE) of sea cucumber fed diet containing 8% Nannochloropsis oculata algae was significantly higher than that of other diets (P<0.05). Results of the experiment suggest that dietary inclusion with 8% Nannochloropsis oculata may improve growth of sea cucumber. The second experiment was conducted to determine IL-10 gene expression where mice splenocytes were stimulated with 10 µg/ml of each diet fed sea cucumber extracts for 2 hours. The result showed that IL-10 gene expression levels were significantly increased in 6% and 8% Nannochloropsis oculata algae containing diets fed sea cucumber extracts. This result suggests that proper combination of Nannochloropsis oculata could up-regulate of IL-10 gene expression. Such detailed information could be helpful in further development of more appropriate diets for sea cucumber culture. Keywords : Sea cucumber (Apostichopus japonicus), Nannochloropsis oculata, growth, interleukin (IL)-10