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¹ College of Fisheries, Central Agricultural University (Imphal), Lembucherra, Agartala–799 210, Tripura, India DOI : 10.4194/1303-2712-v18_3_15 Viewed : 3965 - Downloaded : 3987 A 30-days experiment was conducted to evaluate the efficacy of four chemo-attractants viz. DL-alanine, betaine, Ltryptophan (TRP) and inosine monophosphate (IMP) in the diet of Ompok bimaculatus during fry rearing. Fifty numbers of fifteen days weaned fry (0.0739±0.008 g; 2.32±0.06 cm) were stocked into each aerated aquariums (30.0 x 15.0 x 15.0 cm) following a completely randomized design (CRD) consisting of five treatments including control with three replicates each. Five iso-nitrogenous purified diets were prepared including four treatment diets with attractants (2%) replacing the cellulose and fed to the fishes twice a day. The highest growth was observed in the treatment fed with betaine supplemented diet followed by inosine monophosphate whereas, no significant difference was observed among control, DL-alanine and Ltryptophan supplementation. The highest survivability was found in L-tryptophan supplemented diet (48.66±2.4%) followed by betaine, DL-alanine, control and lowest was found in inosine monophosphate treatment (32.00±2.0). It has also observed that there was significant difference (P>0.05) in survival between L-tryptophan and betaine supplemented diets fed groups. It is concluded that the dietary tryptophan supplementation could be a promising aquaculture management strategy for carnivorous fish as it showed significantly better survival without affecting the growth. Keywords : Ompok bimaculatus, L-tryptophan, betaine, digestive enzyme, inosine monophosphate