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¹ Università degli Studi di Milano, Department of Health, Animal Science and Food Safety Via Trentacoste 2, 20134 Milano, Italy DOI : 10.4194/1303-2712-v17_3_01 Viewed : 4302 - Downloaded : 3409 The aquafeed industry is facing the lack of availability of fish meal and oil. The need of an economic and environmental sustainability has increased the use of plant-derived oils. In order to provide an overview of which ingredients are the most frequently used in GSB diet and as they affect the lipid composition of aquafeeds, we analyzed 26 feed samples collected from farmers. At the same time, 44 farmed GSB were analyzed to compare the fatty acid (FA) composition with 21 wild GSB and 46 GSB farmed in 2005. Twenty-four different ingredients were declared in the analyzed feeds. Fish meal and oil were present in all samples, even if they were not the principal protein and lipid source in the analyzed feeds. If we consider the FA n-3/n-6 ratio, feeds presented a range from the lowest value of 0.35, to the maximum of 2.26. Concerning fish FA, 2014- farmed GSB presented an increased difference with wild GSB, if compared with those reared in 2005, due to the higher presence of oleic and linoleic acid. Our study confirms the increase of use of plant ingredients in aquafeeds and the decrease of the fish meal and oil inclusion in feeds. Keywords : Fish meal and oil replacement, fish feed formulation, aquafeed ingredients