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Turkish Journal of Fisheries and Aquatic Sciences 2018, Vol 18, Num, 1     (Pages: 17-25)

Insulin-Like Androgenic Gland Hormone Gene in the Freshwater Chinese Mitten Crab Eriocheir sinensis:cDNA Cloning, Expression Pattern, and Interaction with EsIGFBP7

Kun Song 1 ,Tianshuo Xu 1 ,Yanan Zang 1 ,Ali Serwadda 1-2 ,Tianhao Dai 1 ,Yuanchao Ma 1 ,Huaishun Shen 1-2

1 Nanjing Agricultural University, Wuxi Fisheries College, Nanjing, 210095, PR China
2 Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Wuxi 214081, PR China
DOI : 10.4194/1303-2712-v18_1_03 Viewed : 448 - Downloaded : 316 Androgenic gland hormone has an insulin-like structure and it is the male-specific hormone found in crustaceans. Androgenic gland hormone controls male sexual differentiation and maintains male sexual characteristics. In this study, the full-length cDNA sequence of the androgenic gland hormone gene in Eriocheir sinensis (EsIAG) was cloned. The total length of the EsIAG cDNA was 1,495 bp, encoding 151 amino acids. EsIAG has the typical conserved structure of other reported IAGs but had low sequence similarity with other crustacean IAGs. EsIAG was expressed specifically in the androgenic gland. Insulin-like growth factor binding protein 7 from the Chinese mitten crab E. sinensis (EsIGFBP7) was highly expressed in the male sex-related organs among nine crab tissues. Interactions between EsIAG and EsIGFBP7 were verified using a yeast-two hybrid assay. These results obtained in this study provide basic information about the regulatory mechanism of male sex differentiation in E. sinensis. Keywords : Eriocheir sinensis, insulin-like androgenic gland hormone, EsIGFBP7, male sexual differentiation