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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 5     (Pages: 399-407)

Isolation, Molecular Identification and The Carotenogenesis Process of the Microalgae Dunaliella salina Strain DunaDZ1 Isolated from an Algerian Salt Lake

Hafsa Yaiche Achour 1 ,Amel Doumandji 2 ,Noureddine Bouras 3-4 ,Nasserdine Sabaou 4 ,Patricia Assunҫão 5

1 Laboratoire de Recherche de Biotechnologie des Productions Végétales, Biotechnology Department, Life Science faculty, Blida 1 University, BP 270, 09000, Blida, Algeria
2 Laboratoire de Recherche de Biotechnologie des Productions Végétales, Agri-Food Department, Life Science faculty, Blida 1 University, BP 270, 9000, Blida, Algeria
3 Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, 47000, Ghardaïa, Algeria
4 Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria
5 Instituto Tecnológico de Canarias (ITC), Departamento de Biotecnología, División de Investigación y DesarrolloTecnológico, Pozo Izquierdo, 35119, Sta. Lucía, Canary Islands, Spain
DOI : 10.4194/1303-2712-v19_5_05 Viewed : 520 - Downloaded : 347 Algeria has a high algal biodiversity, which is of great interest and should be exploited. Isolation of Dunaliellastrain DunaDz1 from SidiAmeur Lake in Algeria was carried out in this study. The aim of this research was to identify the isolated strain by morphological and molecular taxonomy using ITS and rbcL genes. The effect of two stress factors, salinity and nitrate starvation on the carotenoids accumulation in Dunaliella strain was investigated on f/2 and Johnson media. The results for both genes showed that strain DunaDz1 is part of a clade containing several strains of D. salina. The strain grew well in Johnson medium, with a cell number of 56.09 105 cell/ml and a chlorophyll a amount of 63.63 µg/ml. Salt stress lead to a noticeable increase in the total carotenoids levels of strain DunaDz1, 23.68 ± 0.12 and 23.15 ± 0.23 pg/cell, for f/2 and Johnson media, respectively. In nitrate starved media a lesser amount was recorded comparing with salt stress: 11.42 ± 0.22 and 16.2 ± 0.24 pg/cell, for f/2 and Johnson media, respectively. The combination of these two factors was more effective on f/2 medium than Johnson medium. Keywords : Dunaliella, Taxonomy, ITS, rbcL, Carotenoids