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Turkish Journal of Fisheries and Aquatic Sciences 2017, Vol 17, Num, 3     (Pages: 487-498)

Assessment of Antioxidant Biomarkers and Protein Levels in Tissues of Oreochromis mossambicus and Channa punctatus Exposed to Toxicity by Fungicides

Neelanjana Choudhury 1 ,Jayanta Tarafdar 2 ,Ashis Kumar Panigrahi 3

1 Department of Plant Pathology, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia (West Bengal)-741235. India
2 Officer-in-Charge, AICRP- Tuber Crops, Kalyani Centre, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia (West Bengal)-741235. India
3 University of Kalyani, Fishery Extension Laboratory, Department of Zoology, Kalyani, Nadia (West Bengal)-741235. India
DOI : 10.4194/1303-2712-v17_3_05 Viewed : 379 - Downloaded : 195 The present investigation is about change in antioxidant enzymes and protein profile in Oreochromis mossambicus and Channa punctatus due to toxicity induced by application of fungicides in paddy-cum-fish ecosystem in India. Antioxidant enzymes like Lactate dehydrogenase (LDH), Malate dehydrogenase (MDH) and Peroxidase (Pox) are used as biomarkers for toxicological study. Oreochromis mossambicus and Channa punctatus were exposed to different concentration of Azoxystrobin 23% SC and Hexaconazole 5% SC respectively and LC50 was determined. LC50 for Amister was detected to be 0.008 ml/lit and that for Contaf plus was found to be 0.025 ml/lit. In case of Oreochromis mossambicus 1/2.5th, 1/5th and 1/7.5th of LC50 (0.0032 ml/lit, 0.0016 ml/lit and 0.001 ml/lit) were selected for chronic sub-lethal study. For Channa punctatus ½nd, ¼th and 1/6th of LC50 value (0.0125 ml/lit, 0.0062 ml/lit and 0.0041 ml/lit) were chosen. After 3 months of exposure, the individuals exposed to toxicity were sacrificed and stress enzymes and protein profile were checked in gill, heart, liver, kidney, muscle and spleen. Overall result revealed a gradual increase in LDH and Peroxidase but decrement in level of MDH and protein profile was also changed due to toxicity by fungicides. Keywords : Oreochromis mossambicus, Channa punctatus, fungicides, Antioxidant enzymes, Protein profile